Lin28A Rabbit Monoclonal Antibody(ARA838)

Datasheet

MSDS

Lin28A Rabbit Monoclonal Antibody(ARA838)

Key features and details

Target: Lin28A
Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Application: IHC-P, WB
Storage: -20°C

Brand:

CAT.NO. : ARA6653

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

LIN-28 is a highly conserved, RNA-binding, cytoplasmic protein. It consists of a cold shock domain and retroviral-type (CCHC) zinc finger motifs that were first identified in Caenorhabditis elegans. LIN-28 controls the timing of events during embryonic development and is readily expressed in embryos, embryonic stem cells and embryonal carcinoma cells. The presence of LIN-28 persists in some adult tissues including cardiac and skeletal muscle. In differentiating myoblasts, LIN-28 increases protein sysnthesis efficiency and binds to the growth and differentiation factor IGF-II.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:800-1:1,000
IHC	1:400-1:1,000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Name	Lin28A Recombinant Rabbit Monoclonal Antibody (ARA838)
Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within Human Lin 28A aa 10-59 /200.
Species Reactivity	Human, Mouse, Rat
Validated Applications	WB, IHC-P
Molecular Weight	Predicted band size: 23 kDa
Positive Control	NCCIT cell lysates, JAR cell lysate, human testis tissue, mouse placenta tissue, rat testis tissue.
Conjugation	unconjugated

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Lin28A on NCCIT cell lysates with Rabbit anti-Lin28A antibody (ARA838) at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Lin28A antibody (ARA838) at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Lin28A antibody (ARA838) at 1/1,000 dilution.

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-Lin28A antibody (ARA838) at 1/400 dilution.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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