Langerin / CD207 Rabbit Monoclonal Antibody(ARA823)

Datasheet

MSDS

Langerin / CD207 Rabbit Monoclonal Antibody(ARA823)

Key features and details

Target: Langerin / CD207
Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Application: WB, IHC - P, IF
Storage: -20°C

Brand:

CAT.NO. : ARA6615

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Langerin (CD207) is a type II transmembrane protein which is encoded by the CD207 gene in humans. It was discovered by scientists Sem Saeland and Jenny Valladeau as a main part of Birbeck granules. Langerin is C-type lectin receptor on Langerhans cells (LCs) and in mice also on dermal interstitial CD103+ dendritic cells (DC) and on resident CD8+ DC in lymph nodes. Langerin has similar function and structure as a DCs surface protein DC-SIGN (CD209). Both receptors bind similar antigens via the CRD, for instance Mycobacterium tuberculosis and HIV-1. However, whereas HIV-1 binding to langerin leads to the elimination of the virus, HIV-1 binding to DC-SIGN leads to infection of the cell.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:1,00 -1:2,000
IHC	1:500-1:1,000
IF/ICC	1:200-1:5,000
IF/ICC	1:200 - 1:1,000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Immunogen	Synthetic peptide within human Langerin aa 1 - 43 / 328.
Molecular Weight	Predicted band size: 37 kDa
Form/Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Positive Controls	Human lymph nodes tissue, human skin tissue, human tonsil tissue, 293T cells transfected with CD207/Langerin.
Conjugation	unconjugated
Isotype	IgG
Purification	Protein A affinity purified.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Langerin / CD207 on different lysates with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/1,000 dilution.Lane 1: 293T-NT cell lysateLane 2: 293T-OE-CD207/Langerin cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 37 kDaObserved band size: 37 kDaExposure time: 20 seconds; ECL: K1801;4-20% SDS-PAGE gel.

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/4,000 dilution.

Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/4,000 dilution.

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/4,000 dilution.

Immunohistochemical analysis of paraffin-embedded human brain tissue (negative) with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/2,000 dilution.

Immunocytochemistry analysis of 293T cells transfected with or without CD207/Langerin labeling Langerin / CD207 with Rabbit anti-Langerin / CD207 antibody (ARA6615) at 1/2,500 dilution.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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