KLF4 Mouse Monoclonal Antibody(C3524)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:1000 - 1:2000 |
Description | Mouse monoclonal antibody to KLF4 |
Specificity | Recognizes endogenous levels of KLF4 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human KLF4. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 54 kD; Observed: 62 kD |
Form/Buffer | Mouse IgG2b kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | EZF; GKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor |
Gene Symbol | KLF4 |
Entrez Gene | 9314(Human) |
SwissProt | O43474(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of KLF4 expression in recombincant protein (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 62 kD)
Immunofluorescent analysis of KLF4 staining in Hela cells transfected with pMX constructs. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Hela cells using Anti-KLF4 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 594 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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