Ki-67 Rabbit Monoclonal Antibody(ARA786)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
IHC | 1:200-1:400 |
IF/ICC | 1:100-1:1000 |
FC | 1:100-1:500 |
Description | Rabbit Monoclonal Antibody to Ki-67 |
Antibody Type | Primary antibody |
Predicted MW | 359kDa |
Immunogen | A synthetic peptide corresponding to Ki-67 residues Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) within aa1000-1200 of Ki-67 was used as an analysis of Endometrium cancer tissue labelling Ki-67 with RR677 at 1:400. immunogen. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.61%. |
Alternative Names | Antigen KI-67 |
Gene Symbol | MKI67 |
Entrez Gene | 4288(Human) |
Swissprot | P46013 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Endometrium cancer tissue labelling Ki-67 with ARA786 at 1:400. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Overlay histogram showing Ramos cells stained with ARA786 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA786, 1:100 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
ARA786 staining Ki-67 in Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:1,000) at 4°C. An Alexa Fluor<sup>®</sup> 594-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor<sup>®</sup> 594-conjugated Goat Anti-Rabbit IgG(1:500).
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