KDM5C Mouse Monoclonal Antibody(C3273)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
Description | Mouse monoclonal antibody to KDM5C |
Specificity | Recognizes endogenous levels of KDM5C protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human KDM5C / Jarid1C / SMCX protein fragments expressed in E.coli |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 176 kD; Observed: 220 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | DXS1272E; JARID1C; SMCX; XE169; Lysine-specific demethylase 5C; Histone demethylase JARID1C; Jumonji/ARID domain-containing protein 1C; Protein SmcX; Protein Xe169 |
Gene Symbol | KDM5C |
Entrez Gene | 8242(Human) |
SwissProt | P41229(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of KDM5C expression in Hela (A), 293T (B), T47D (C), MCF7 (D) whole cell lysates. (Predicted band size: 176 kD; Observed band size: 220 kD)
Immunofluorescent analysis of KDM5C staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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