IKK alpha Rabbit Monoclonal Antibody(C3093)

MSDS

IKK alpha Rabbit Monoclonal Antibody(C3093)

Key features and details

Rabbit monoclonal antibody to IKK alpha

Target: IKK alpha
Source/Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: WB, IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02705

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses . Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on serine residues . These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome . In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IP	1:10 - 1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit monoclonal antibody to IKK alpha
Specificity	Recognizes endogenous levels of IKK alpha protein.
Antibody Type	Primary antibody
Imnunogen	A synthetic peptide of human IKK alpha
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 85 kD; Observed: 85 kD
Form/Buffer	Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA.
Alternative Names	IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16
Gene Symbol	CHUK
Entrez Gene	1147(Human)
SwissProt	O15111(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of IKK alpha expression in Jurkat (A), Hela (B) whole cell lysates. (Predicted band size: 85 kD; Observed band size: 85 kD)

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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