Human pTau181 antibody pair (ARA989/ARA971)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA6880
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Product Details
Background
Plasma phosphorylated tau 181 (p-tau181) has emerged as a promising biomarker for Alzheimer's disease (AD) and related conditions. Studies show that elevated plasma p-tau181 levels are associated with increased amyloid-beta (Aβ) deposits across various brain regions in cognitively healthy adults. Furthermore, p-tau181 levels correlate with Aβ-positivity on positron emission tomography (PET), and they predict the conversion to dementia stage depending on renal function. However, p-tau181 does not appear to be directly linked to tau-PET burden.Additionally, plasma p-tau181 levels have been associated with cognitive decline in various populations, such as those with AD, mild cognitive impairment (MCI), and other neurodegenerative diseases. Moreover, p-tau181 levels have been identified as a noninvasive diagnostic and prognostic tool in clinical practice and trials.
Application
The pTau181 Matched Antibody Pair is expertly designed for immunoassay technologies and high-throughput ELISA platforms that require specifically labeled antibody pairs. These pairs can be customized with a variety of labels such as fluorophores, biotin, and beads. Compatible technologies include MSD, Luminex, Quanterix Simoa, Alpha Technology etc.*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Overview
Antibody Pair Specificity | pTau181 |
Species Reactivity | Human |
Protein Standard | Recombinant Human p-Tau181 Protein |
Target Antigen | Protein name: Microtubule-associated protein tau; Gene name: MAPT; Organism: Homo sapiens (Human) |
Cross Reactivity | Not cross-react with Tau peptide or recombinant proteins without any phosphorylation site. |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Sandwich ELISA Microtiter wells were coated with ARA6887 at 1 µg/mL as the capture antibody. Recommbinant pTau181 protein (AXP6613) was used as the antigen. 400 ng/mL biotin conjugated Tau Antibody ARA6837 was used as the detection antibody. Result: ARA6887 and ARA6837 can be used as a matched antibody pair to detect and quantify the concentration of Human pTau181 with high specificity.
Component
| Products include | Catalog No. | Isotype | Quantity | Form |
|---|---|---|---|---|
| pTau181 Antibody （ARA6887） | ARA972 | Mouse IgG2b | 100μg | Liquid |
| Tau Antibody (ARA6837) | ARA958 | Mouse IgG2b | 100μg | Liquid |
| Recombinant Human p-Tau181 Protein | AXP6613 | / | 20μg | Liquid |
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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