Human MMP-13 ELISA

Protocol Booklet

Key features and details

Species: Human
Size: 5 plates,15 plates,50 plates
Sample Volume: 50 μl
Standard Curve Range: 93.75pg/ml-6000pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 9.23 pg/ml(50 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEH0135

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

MMP13 (matrix metallopeptidase 13) participates in tissue remodeling, inflammation, cytokine secretion and activation. It also plays an important role in acute lung injury, viral infections and chronic obstructive pulmonary disease. Mutation in MMP13 results in spondyloepimetaphyseal dysplasia, missouri type (SEMDMO).

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0787	0.0758	0.0773
78.13	0.1224	0.1179	0.1202	0.0429
156.25	0.1726	0.1656	0.1691	0.0919
312.50	0.2574	0.2588	0.2581	0.1809
625.00	0.3896	0.3781	0.3839	0.3066
1250.00	0.6900	0.6888	0.6894	0.6122
2500.00	1.2610	1.1250	1.1930	1.1158
5000.00	2.3310	2.2210	2.2760	2.1988

product features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

kit content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

other reagents required

ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.

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