Human Lipoprotein A/LPA ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 1.11 pg/ml (50 μl)；7.76 pg/ml (10 μl)；
Standard Curve Range: 13.72~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0171

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Size:

96T

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Product Details

Background

The protein encoded by this gene is a serine proteinase that inhibits the activity of tissue-type plasminogen activator I. The encoded protein constitutes a substantial portion of lipoprotein(a) and is proteolytically cleaved, resulting in fragments that attach to atherosclerotic lesions and promote thrombogenesis. Elevated plasma levels of this protein are linked to atherosclerosis. Depending on the individual, the encoded protein contains 2-43 copies of kringle-type domains. The allele represented here contains 15 copies of the kringle-type repeats and corresponds to that found in the reference genome sequence.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0070	0.0063	0.0067
13.72	0.0173	0.0213	0.0193	0.0127
41.15	0.0419	0.0407	0.0413	0.0347
123.46	0.1194	0.1158	0.1176	0.1110
370.37	0.3628	0.3434	0.3531	0.3465
1111.11	1.0670	1.0320	1.0495	1.0429
3333.33	2.5740	2.5650	2.5695	2.5629
10000.00	3.9935	4.0450	4.0193	4.0126

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	375.0	1408.8	3317.3	371.3	1405.8	3425.6
Standard Deviation	10.0	36.1	99.1	12.8	69.1	226.0
Coefficient of Variation(%)	2.7	2.6	3.0	3.4	4.9	6.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Lipoprotein A/LPA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 118% with an overall mean recovery of 98%.

Sample Values

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	288.08-11292.84	100	2363.67

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Lipoprotein A/LPA in this assay. No medical histories were available for the donors.

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