Human Leptin ARExSet® antibody pair for ELISA

Protocol Booklet

Human Leptin ARExSet® antibody pair for ELISA

Key features and details

Species: Human
Size: 5 plates,15 plates,50 plates
Sample Volume: 50 μl
Standard Curve Range: 31.25 pg/ml-2000 pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 0.42 pg/ml(50 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEH0056

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Leptin (anti-obesity protein) is a protein product of the mouse ob (obesity) gene and appears to be involved in appetite control. The ob gene is expressed in adipose tissue and is believed to regulate fat storage in the human body. Mice with ob/ob genotype also develop a form of diabetes similar to type II (non-insulin-dependent) diabetes. Mouse leptin is a 16 kDa protein containing 147 amino acid residues.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0405	0.0421	0.0413
31.25	0.1459	0.1363	0.1411	0.0998
62.50	0.2217	0.2166	0.2192	0.1779
125.00	0.3683	0.3594	0.3639	0.3226
250.00	0.7537	0.6959	0.7248	0.6835
500.00	1.1770	1.1870	1.1820	1.1407
1000.00	2.0620	2.0120	2.0370	1.9957
2000.00	3.3900	3.2800	3.3350	3.2937

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet^®Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01.

Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS5, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA, 0.05%Tween20, PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5 mol/ml H₂SO₄. AREX Biosciences, Catalog # DSSS01.

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