Human IL-23 p19 ELISA Kit

Protocol Booklet

Key features and details

Species: Human
Size: 96T
Sample Volume: 50 μl
Standard Curve Range: 1.37~1000 pg/ml
Assay type: Fully Ready-to-Use
Sensitivity: 0.32 pg/ml (50 μl)；1.3 pg/ml (10 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : AEH0109

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Size:

96T

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Product Details

Background

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Mouse p19 cDNA encodes a 196 amino acid (aa) residue precursor protein with a putative 19 aa signal peptide and 177 aa mature protein. Human and mouse p19 share 70% aa sequence identity. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R). IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both na茂ve and memory human T cells, the effects of IL-23 is restricted to memory T cells. In mouse, IL-23 but not IL-12, has also been shown to induce memory T cells to secret IL-17, a potent proinflammatory cytokine. IL-12 and IL-23 can induce IL-12 production from mouse splenic DC of both the CD8- and CD8+ subtypes, however only IL-23 can act directly on CD8+ DC to mediate immunogenic presentation of poorly immunogenic tumor/self peptide

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0146	0.0163	0.0155
1.37	0.0224	0.0216	0.0220	0.0066
4.12	0.0360	0.0386	0.0373	0.0219
12.35	0.0823	0.0756	0.0790	0.0635
37.04	0.2068	0.2039	0.2054	0.1899
111.11	0.5727	0.5620	0.5674	0.5519
333.33	1.4960	1.4830	1.4895	1.4741
1000.00	3.4620	3.3150	3.3885	3.3731

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	21.7	101.7	296.8	20.6	102.9	318.1
Standard deviation	1.0	3.1	26.8	1.0	3.1	11.3
Coefficient of variation（%）	4.4	3.0	4.2	5.1	3.0	3.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike recovery

The spike recovery was evaluated by spiking 3 levels of human IL-23 p19 into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 81% to 112% with an overall mean recovery of 96%.

Sample values

Sample Matrix

Sample Evaluated

Range (pg/ml)

Detectable

Mean of Detectable (pg/ml)

Serum

n.d.-6.26

0.69

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-23 p19 in this assay. No medical histories were available for the donors.

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