Human IL-13R alpha 2 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0182
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Product Details
Background
Two members of the type 5 subfamily of type I cytokine receptors can serve as receptors for IL-13. IL-13 can bind to IL-13 R alpha 1 (CD213a1; previously designated IL-13 R alpha or NR4) with low affinity, then recruits the IL-4 R alpha chain to form a high affinity receptor, causing downstream STAT6 activation. Alternately, IL-13 can bind IL-13 R alpha 2 (CD213a2) with high affinity; this interaction does not cause activation of STAT6, but does induce TGF-beta production. IL-13 R alpha 1 and IL-13 R alpha 2 each have three extracellular fibronectin type III domains, two cytokine receptor homology modules and a WSXWS motif typical of the class I cytokine receptor family, but IL-13 R alpha 2 has a much shorter cytoplasmic tail. IL-13 R subunits can be expressed on monocytes, macrophages, fibroblasts, human B cells, basophils, eosinophils, endothelial cells, and smooth muscle cells.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0106 |
0.0100 |
0.0103 |
|
|
27.43 |
0.0238 |
0.0228 |
0.0233 |
0.0130 |
|
82.30 |
0.0507 |
0.0475 |
0.0491 |
0.0388 |
|
246.91 |
0.1314 |
0.1236 |
0.1275 |
0.1172 |
|
740.74 |
0.3798 |
0.3780 |
0.3789 |
0.3686 |
|
2222.22 |
1.0320 |
1.0130 |
1.0225 |
1.0122 |
|
6666.67 |
2.5480 |
2.5080 |
2.5280 |
2.5177 |
|
20000.00 |
4.1779 |
4.1531 |
4.1655 |
4.1552 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
372.5 |
1840.8 |
6053.0 |
379.6 |
1922.9 |
6355.7 |
|
Standard Deviation |
20.6 |
126.5 |
290.5 |
18.2 |
110.3 |
206.8 |
|
Coefficient of Variation(%) |
5.5 |
6.9 |
4.8 |
4.8 |
5.7 |
3.3 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-13 R alpha 2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 105% with an overall mean recovery of 87%.
The recovery ranged from 80% to 105% with an overall mean recovery of 87%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-23.65 | 60 | 8.9 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-13 R alpha 2 in this assay. No medical histories were available for the donors.
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