Human GITR-TNFRSF18 ARExSet® antibody pair for ELISA
Protocol Booklet 
Key features and details
- Species:
- Size:
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- Sensitivity:
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Brand:
CAT.NO. : ASEH0095
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Product Details
Background
GITR-TNFRSF18, also known as glucocorticoid-induced tumor necrosis factor receptor (GITR), is a transmembrane protein that plays a role in immune regulation:
Structure
GITR is a type I transmembrane glycoprotein that is a member of the tumor necrosis factor receptor superfamily (TNFRSF). It is encoded by the tnfrsf18 gene on chromosome 4 in mice.
Expression
GITR is expressed in several immune cells, including B lymphocytes, effector T cells, NK cells, eosinophils, macrophages, and activated DCs.
Function
GITR activation can promote the function of effector T cells, inhibit the function of Treg cells, and increase T9 differentiation. GITR triggering can also induce both pro- and anti-apoptotic effects.
Role in disease
GITR activation can cause the development of autoimmune diseases. GITR knockout mice have lower mortality in an ischemia model.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0330 | 0.0312 | 0.0321 | |
| 62.5 | 0.1179 | 0.0970 | 0.1075 | 0.0754 |
| 125 | 0.2039 | 0.1749 | 0.1894 | 0.1573 |
| 250.00 | 0.3421 | 0.3216 | 0.3319 | 0.2998 |
| 500.00 | 0.6266 | 0.5629 | 0.5948 | 0.5627 |
| 1000.00 | 1.1260 | 1.0880 | 1.1070 | 1.0749 |
| 2000.00 | 2.0870 | 2.0140 | 2.0505 | 2.0184 |
| 4000.00 | 3.6800 | 3.6860 | 3.6830 | 3.6509 |
Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
ARExSet® Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.
- 96 well microplates: AREX Biosciences, Catalog # DSEP01. Plate Sealers: AREX Biosciences, Catalog # DSSF01.
- Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2-7.4, 0.2 μm fi ltered . AREX Biosciences, Catalog # DSCB01.
- Blocking Buffer: AREX Biosciences, Catalog # DSBB01.
- Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.
- Assay Buffer: 0.5%BSA，0.05%Tween20，PBS Solution.AREX Biosciences, Catalog # DSAB01
- Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.
- Stop Solution: 0.5mol/ml H2SO4. AREX Biosciences, Catalog # DSSS01.
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