Human CXCL6/GCP-2 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0165
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Product Details
Background
GCP-2 (granulocyte chemotactic protein-2) is a CXC chemokine. Among human CXC chemokines, GCP-2 is most closely related to ENA-78. The structure and sequence of the genes for human GCP-2 and ENA-78 also exhibit close similarity, suggesting the two genes may have originated from a recent gene duplication. LIX (LPS-induced CXC chemokine) was initially cloned as a gene induced by LPS in mouse fibroblasts. The mouse protein designated GCP-2, because of its amino acid sequence similarity (60%) to human GCP-2, is identical to the LIX protein sequence.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0073 |
0.0077 |
0.0075 |
|
|
31.25 |
0.0185 |
0.0197 |
0.0191 |
0.0116 |
|
62.50 |
0.0450 |
0.0437 |
0.0444 |
0.0369 |
|
125.00 |
0.1488 |
0.1519 |
0.1504 |
0.1429 |
|
250.00 |
0.5995 |
0.6087 |
0.6041 |
0.5966 |
|
500.00 |
1.6640 |
1.7040 |
1.6840 |
1.6765 |
|
1000.00 |
2.9450 |
2.9480 |
2.9465 |
2.9390 |
|
2000.00 |
3.8530 |
3.8660 |
3.8595 |
3.8520 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
139.4 |
425.2 |
854.1 |
107.3 |
323.6 |
710.4 |
|
Standard Deviation |
6.7 |
8.8 |
16.6 |
3.6 |
19.4 |
26.5 |
|
Coefficient of Variation(%) |
4.8 |
2.1 |
1.9 |
3.4 |
6.0 |
3.7 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human CXCL6/GCP-2 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 118% with an overall mean recovery of 108%.
The recovery ranged from 90% to 118% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 18.88-92.95 | 100 | 55.65 |
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