Human CKMB ARExSet® antibody pair for ELISA

Protocol Booklet

Human CKMB ARExSet® antibody pair for ELISA

Key features and details

Species: Human
Size: 5 plates,15 plates,50 plates
Sample Volume: 50 μl
Standard Curve Range: 0.31ng/ml-20ng/ml
Assay type: Not Ready-to-Use
Sensitivity: 5.10pg/ml(50 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEH0072

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

The creatine kinase molecule is a dimer composed of a brain-type subunit (B) and a muscle-type subunit (M). There are usually three isozymes in normal human tissues, which are CK-BB (CK1), CK-MB (CK2) and CK-MM (CK3) in the order of electrophoresis. CK-BB is mainly found in smooth muscle and brain tissue, and almost none in serum; CK-MB mainly exists in myocardial tissue, accounting for 0~4% of CK in serum; CK-MM is mainly present in skeletal muscle, accounting for 96%~100% of CK in serum. CK-MB has its own subtypes, including MB2 (tissue form) and MB1 (transformed form). After the release of CK-MB2 from the myocardium, the carboxy-terminus is hydrolyzed by plasma carboxypeptidase N, and a lysine residue is removed to be converted to the serum-modified isoform CK-MB1. CK and CK-MB are one of the sensitive indicators of myocardial tissue injury, especially CK-MB is more specific. The increase in CK during myocardial injury is mainly due to the increase in CK-MB, which is manifested by the simultaneous increase of both.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0110	0.0121	0.0116
0.31	0.0777	0.0777	0.0777	0.0662
0.63	0.1239	0.1433	0.1336	0.1221
1.25	0.2364	0.2788	0.2576	0.2461
2.50	0.4757	0.5085	0.4921	0.4806
5.00	0.9176	0.9716	0.9446	0.9331
10.00	1.6840	1.7730	1.7285	1.7170
20.00	2.8310	2.7920	2.8115	2.8000

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet^®Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01.

Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS5, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA, 0.05%Tween20, PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5 mol/ml H₂SO₄. AREX Biosciences, Catalog # DSSS01.

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