Human B7-H6/NCR3LG1 ARExSet® antibody pair for ELISA

Protocol Booklet

Human B7-H6/NCR3LG1 ARExSet® antibody pair for ELISA

Key features and details

Species: Human
Size: 5 plates,15 plates,50 plates
Sample Volume: 50 μl
Standard Curve Range: 78.13pg/ml-5000pg/ml
Assay type: Not Ready-to-Use
Sensitivity: 13.41 pg/ml(50 μl)
Operation Duration: 120min
Assay type: Sandwich Elisa

Brand:

CAT.NO. : ASEH0116

US$ Please choose

Size:

5 plates 15 plates 50 plates

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. Within the ECD, human B7-H6 shares 99%, 94%, and 87% aa sequence identity with chimpanzee, orangutan, and gibbon B7-H6, respectively, and 53% - 56% with bovine, canine, and equine B7-H6. Orthologs in mouse and rat have not been identified. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors. The expression of NKp30 ligands on tumor cells correlates with tumor cell sensitivity to NKp30‑dependent cell lysis.

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0197	0.0185	0.0191
78.13	0.0326	0.0290	0.0308	0.0117
156.25	0.0353	0.0342	0.0348	0.0157
312.50	0.0694	0.0674	0.0684	0.0493
625.00	0.1357	0.1303	0.1330	0.1139
1250.00	0.3291	0.2982	0.3137	0.2946
2500.00	1.1440	1.1820	1.1630	1.1439
5000.00	3.7650	3.7500	3.7575	3.7384

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

ARExSet^®Ancillary Reagent Kit (5 plates): containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and assay buffer.

96 well microplates: AREX Biosciences, Catalog # DSEP01.

Plate Sealers: AREX Biosciences, Catalog # DSSF01.

Coating Buffer: 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2-7.4, 0.2 μm filtered . AREX Biosciences, Catalog # DSCB01.

Blocking Buffer: AREX Biosciences, Catalog # DSBB01.

Wash Buffer: 0.05% Tween 20 in PBS, pH 7.2-7.4. AREX Biosciences, Catalog # DSWB01.

Assay Buffer: 0.5%BSA, 0.05%Tween20, PBS Solution.AREX Biosciences, Catalog # DSAB01

Substrate Solution: Tetramethylbenzidine. AREX Biosciences, Catalog # DSTS01.

Stop Solution: 0.5 mol/ml H₂SO₄. AREX Biosciences, Catalog # DSSS01.

New Products

hCG alpha Mouse Monoclonal Antibody(ARA629)

E.coli Residual Total RNA Detection Kit (RT-PCR)

Fibronectin Rabbit Monoclonal Antibody(C6)

Antigen Retrieval Solution II (EDTA)

Antigen Retrieval Solution I (Citrate)

Hyaluronic Acid ELISA Kit