Horseradish Peroxidase (HRP) Rabbit Monoclonal Antibody(ARA805)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
-
Brand:
Product Details
WB | 1:5000-1:10000 |
IF/ICC | 1:100-1:200 |
FC | 1:20-1:50 |
Description | Rabbit Monoclonal Antibody to Horseradish Peroxidase (HRP) |
Antibody Type | Primary antibody |
Predicted MW | 39kDa |
Immunogen | Full length native protein (purified) corresponding to HRP (Horseradish). |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.80%. |
Alternative Names | Horseradish Peroxidase;HPRC1; PRXC1A; Peroxidase C1A;HRP |
Swissprot | P00433 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
All lanes: Anti-HRP antibody at 1:10,000 dilution
Predicted MW: 39 kDa
Observed MW: 39 kDa
Lane 1: HRP protein
Lysate at 20 ng per lane
2nd Ab:
GAR HRP(H+L) 1:5,000
Exposure: 60s
ARA805 staining HRP in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with anti-actin (RR608) antibody (1:200) conjugated to HRP at 4°C. FITC-conjugated ARA805 was used as the secondary antibody (1:50). DAPI (blue) was used as the nuclear counter stain.
Control: primary antibody, anti-actin (RR608) antibody (1:200) and PBS.
Overlay histogram showing Hela cells stained with ARA805 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (RR608, 1:50 dilution) conjugated to HRP in 1x PBS/1% BSA for 60 min at room temperature. The secondary antibody used was ARA805 conjugated with FITC at 1:50 dilution for 60 min at room temperature. Unlabelled sample (Black) was used as a control.
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