Histone H4 (MonoMethyl-K20) Mouse Monoclonal Antibody(C2737)

Datasheet

MSDS

Histone H4 (MonoMethyl-K20) Mouse Monoclonal Antibody(C2737)

Key features and details

Mouse monoclonal to Histone H4 (MonoMethyl-K20)

Target: Histone H4 (MonoMethyl-K20)
Source/Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Applications: WB, IHC, IF/ICC, FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA02349

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:100 - 1:500
IF/ICC	1:100 - 1:500
FC	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal to Histone H4 (MonoMethyl-K20)
Specificity	Recognizes endogenous levels of Histone H4 (MonoMethyl-K20) protein
Antibody Type	Primary antibody
Imnunogen	Synthesized peptide of human Histone H4 (MonoMethyl-K20)
Purification	This antibody is purified through a protein G column.
Molecular Weight	Predicted: 11 kD; Observed: 14 kD kD
Form/Buffer	Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	H4/A; Histone H4
Gene Symbol	HIST1H4A
Entrez Gene	8370(Human)
SwissProt	P62805(Human)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Histone H4 (MonoMethyl-K20) expression in THP1 (A) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 14 kD kD)

Immunohistochemical analysis of Histone H4 (MonoMethyl-K20) staining in human rectum cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H4 (MonoMethyl-K20) staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Raji cells using Anti-Histone H4 (MonoMethyl-K20) Antibody (green) and negative control (red).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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