HA-tag Mouse Monoclonal Antibody(C2044)

MSDS

Key features and details

Mouse monoclonal antibody to HA-tag

Target: HA-tag
Source/Host: Mouse
Reactivity: N/A
Clonality: Monoclonal
Applications: WB, IF/ICC, IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA01656

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

The HA-tag is a nine-amino-acid peptide (YPYDVPDYA) derived from the human influenza hemagglutinin (HA) protein. It is one of the most widely used epitope tags for detection, immunoprecipitation, and purification of recombinant proteins. The tag does not appear to interfere with the bioactivity or biodistribution of recombinant proteins, and HA-tag antibodies are commonly used in Western blot, IHC, IF, ChIP, and FACS applications.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000 - 1:5000
IF/ICC	1:200 - 1:500
IP	1:100 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal antibody to HA-tag
Specificity	Recognizes C-terminal, internal, and N-terminal HA-tag fusion proteins.
Antibody Type	Primary antibody,Tag
Imnunogen	KLH-conjugated synthetic peptide encompassing a sequence of HA-tag. The exact sequence is proprietary.
Purification	Affinity chromatography
Molecular Weight	N/A
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names
Gene Symbol
Entrez Gene
SwissProt

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of over-expressed HA-tagged protein in 293T cell lysate.

Immunofluorescent analysis of HA-tag staining in 293T cells transfected with a HA-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation of HA-tagged protein from HEK293T cells transfected with vector overexpressing HA tag, using Anti-HA-tag Antibody.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for use in diagnostic procedures.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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