GSK3 beta Mouse Monoclonal Antibody(C3444)

Datasheet

MSDS

GSK3 beta Mouse Monoclonal Antibody(C3444)

Key features and details

Mouse monoclonal antibody to GSK3 beta

Target: GSK3 beta
Source/Host: Mouse
Reactivity: Human,Mouse,Rat
Clonality: Monoclonal
Applications: WB, IHC, FC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : AMA03056

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1 , EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1 . Requires primed phosphorylation of the majority of its substrates . In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis . May also mediate the development of insulin resistance by regulating activation of transcription factors .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:1000
IHC	1:50 - 1:100
FC	1:50 - 1:100

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Mouse monoclonal antibody to GSK3 beta
Specificity	Recognizes endogenous levels of GSK3 beta protein.
Antibody Type	Primary antibody
Imnunogen	Purified recombinant fragment of human GSK3B (AA: 2-159) expressed in E. Coli.
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 47 kD; Observed: 47 kD
Form/Buffer	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
Alternative Names	Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
Gene Symbol	GSK3B
Entrez Gene	2932(Human); 56637(Mouse); 84027(Rat)
SwissProt	P49841(Human); Q9WV60(Mouse); P18266(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of GSK3 beta expression in Hela (A), NIH3T3 (B), C6 (C), rat brain (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunohistochemical analysis of GSK3 beta staining in human esophageal cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Flow cytometric analysis of GSK3 beta in NIH3T3 cells (green) and negative control (red).

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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