GRP78 Mouse Monoclonal Antibody(C3451)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
IP | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to GRP78 |
Specificity | Recognizes endogenous levels of GRP78 protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human BiP/GRP78 protein expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 72 kD; Observed: 78 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP |
Gene Symbol | HSPA5 |
Entrez Gene | 3309(Human); 14828(Mouse); 25617(Rat) |
SwissProt | P11021(Human); P20029(Mouse); P06761(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of GRP78 expression in NIH3T3 (A), A549 (B), C6 (C), Hela (D) whole cell lysates. (Predicted band size: 72 kD; Observed band size: 78 kD)
Immunofluorescent analysis of GRP78 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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