GPR49 Mouse Monoclonal Antibody(C3538)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
FC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to GPR49 |
Specificity | Recognizes endogenous levels of GPR49 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR49. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 99 kD; Observed: 110 kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | GPR49; GPR67; Leucine-rich repeat-containing G-protein coupled receptor 5; G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38 |
Gene Symbol | LGR5 |
Entrez Gene | 8549(Human) |
SwissProt | O75473(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of GPR49 expression in K562 (A) whole cell lysates. (Predicted band size: 99 kD; Observed band size: 110 kD)
Flow cytometric analysis of Hela cells using Anti-GPR49 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AREX® Fluor 488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.
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