Glypican-3 Rabbit Monoclonal Antibody(ARB961)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6753
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Size:
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Product Details
Background
Glypican 3, also known as GPC3, is a human gene. The protein encoded by this gene is a member of the glypican family. Cell surface heparan sulfate proteoglycans are composed of a membrane - associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican - related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl - phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. Glypican - 3 antibody has been identified to be a useful tumor marker for the diagnosis of Hepatocellular Carcinoma (HCC). Hepatoblastoma, Melanoma, Testicular Germ Cell Tumors, and Wilms Tumor. In patients with HCC, GPC3 was overexpressed in neoplastic liver tissue and detected in serum but was undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression was also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. Glypican - 3 antibody is mainly used in clinical diagnosis of hepatocellular carcinoma.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 66 kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Cytoplasm |
Swissprot ID | P51654 |
Immunogen | Synthetic peptide of Glypican-3 |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05% |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18° C-25° C) for 30 minutes. |
Alternative Names | GPC3, OCI-5 |
Gene Symbol | GPC3 |
Entrez Gene | 2717 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human liver carcinoma tissue sections using Glypican-3 Rabbit Monoclonal Antibody (ARB961).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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