GGT1 Mouse Monoclonal Antibody (F-7)

Datasheet

MSDS

Key features and details

Target: GGT1
Host: Mouse
Reactivity: Mouse, Rat
Clonality: Monoclonal
Application: WB, IP, IF, IHC(P) , ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA0107

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

GGT (γ - glutamyltranspeptidase) acts as a glutathionase and catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. This enzyme is located on the outer surface of the cell membrane and is widely distributed in mammalian tissues involved in absorption and secretion. In humans, hepatic GGT activity is elevated in some liver diseases. GGT1 is released into the bloodstream after liver damage, and an elevated level of the enzyme may be a useful early sign of hepatocellular carcinoma. GGT5 converts leukotriene C4 to leukotriene D4; it does not, however, convert synthetic substrates that are commonly used to assay GGT. In human serum and in human tissues, there is a marked heterogeneity in GGT, but this heterogeneity can be attributed to different glycosylation of the same peptide rather than to the products of different genes.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:100-1:1000
IHC	1:50-1:500
IF/ICC	1:50-1:500
ELISA	1:30-1:3000
IP	1-2 µg/100-500 µg total protein

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Isotype	Mouse IgG2b kappa light chain
Target Antigen	GGT1 (Gamma-glutamyltranspeptidase 1 heavy chain)
Gene Name	GGT1
UniProt ID	P19440 (Human), Q60928 (Mouse), P09480 (Rat)
Entrez Gene ID	2678 (Human), 14598 (Mouse), 25283 (Rat)
Molecular Weight	64 kDa
Gene Aliases	GGT, GGT 1, Gamma-glutamyltransferase 1, Glutathione hydrolase 1
Immunogen	amino acids 351-389 mapping within an internal region of GGT1 of mouse origin
Form/Buffer	PBS with < 0.1% sodium azide and 0.1% gelatin

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of GGT1 expression in non - transfected (A) and mouse GGT1 transfected (B) 293T whole cell lysates.

Immunoperoxidase staining of formalin fixed, paraffin-embedded rat urinary bladder tissue showing cytoplasmic staining of urothelial cells.

Immunoperoxidase staining of formalin fixed, paraffin-embedded rat kidney tissue showing apical membrane and cytoplasmic staining of cells in tubules.

Storage

Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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