Gata3 Mouse Monoclonal Antibody(ARA639)

Datasheet

MSDS

Key features and details

Target: Gata3
Clone ID: ARA639
Source/Host: Mouse
Reactivity: Human
Clonality: Monoclonal
Application: IHC
Dilution: 1:500-1:1000
Storage: -20°C

Brand:

CAT.NO. : ARA6343

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Size:

1mL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

GATA3 is a transcription factor that in humans is encoded by the GATA3 gene. Studies in animal models and humans indicate that it controls the expression of a wide range of biologically and clinically important genes. The GATA3 transcription factor is critical for the embryonic development of various tissues as well as for inflammatory and humoral immune responses and the proper functioning of the endothelium of blood vessels. GATA3 haploinsufficiency (i.e. lose of one or the two inherited GATA3 genes) results in a congenital disorder termed the Barakat syndrome. Current clinical and laboratory research is focusing on determining the benefits of directly or indirectly blocking the action of GATA3 in inflammatory and allergic diseases such as asthma. It is also proposed to be a clinically important marker for various types of cancer, particularly those of the breast. However, the role, if any, of GATA3 in the development of these cancers is under study and remains unclear.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
IHC	1:500-1:1000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Type	Primary antibodies
Isotype	IgG2c
Positive Control	MCF7 cell lysate, Jurkat cell lysate, human breast carcinoma tissue, human breast tissue, human kidney tissue, SH-SY5Y.
Localization	Nucleus.
Form/Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purification	Protein A affinity purified.
Alternative Names	GATA 3 antibody; GATA binding factor 3 antibody; GATA binding protein 3 antibody; GATA-binding factor 3 antibody; Gata3 antibody; GATA3_HUMAN antibody; HDR antibody; HDRS antibody; MGC2346 antibody; MGC5199 antibody; MGC5445 antibody; Trans acting T cell specific transcription factor GATA 3 antibody; Trans-acting T-cell-specific transcription factor GATA-3 antibody
Molecular Weight	Predicted band size: 48 kDa
Imnunogen	Synthetic peptide within Human GATA3 aa 224-373.
UniProt	P23771(Human);

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue with Gata3 Mouse Monoclonal Antibody(ARA639).

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Gata3 Mouse Monoclonal Antibody(ARA639).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?

AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.

What development scenarios are pathology raw antibodies mainly used for?

Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.

How can I evaluate whether a raw antibody is suitable for our staining platform?

It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.

Can you provide samples for platform validation?

Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.

How should raw antibodies be paired with detection systems?

AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.

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