FUBP3 Mouse Monoclonal Antibody(C3972)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to FUBP3 |
Specificity | Recognizes endogenous levels of FUBP3 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human FUBP3. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 61 kD; Observed: 62 kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | FBP3; Far upstream element-binding protein 3; FUSE-binding protein 3 |
Gene Symbol | FUBP3 |
Entrez Gene | 8939(Human) |
SwissProt | Q96I24(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of FUBP3 expression in Hela (A), U251 (B) whole cell lysates. (Predicted band size: 61 kD; Observed band size: 62 kD)
Immunofluorescent analysis of FUBP3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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