FPN1/Ferroportin/SLC40A1 Rabbit Polyclonal Antibody

Datasheet

MSDS

FPN1/Ferroportin/SLC40A1 Rabbit Polyclonal Antibody

Key features and details

Target: FPN1/Ferroportin/SLC40A1
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Polyclonal
Application: WB, IHC, IF, FCM, ELISA
Storage: -20°C

Brand:

CAT.NO. : ARA6618

US$ Please choose

Size:

20μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Ferroportin (FPN1 or SLC40A1) is the primary protein responsible for iron export from cells in mammals. It transports iron from the inside of a cell to the outside, and is essential for maintaining iron balance in the body. Disruptions in FPN1 function can lead to iron overload or deficiency.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:500-2000
IHC	1:50-400
IF/ICC	1:50-400
FC	1-3 μg/1x106 cells

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Species Reactivity	Human, Mouse, Rat
Application	WB, IHC, IF, FCM, ELISA
Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Conjugate	Unconjugated
Immunogen	Peptide (C)EGTHLMGVKDSNIHE, corresponding to amino acid residues 261-275 of human Ferroportin . 2nd intracellular loop. Note: the structure of Ferroportin is still controversial and there is no defined topology yet.
Form	Liquid
Purification	Antigen affinity chromatography
Form/Buffer	PBS, pH 7.4, 1% BSA.
Contains	0.05% sodium azide

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of rat spleen (lanes 1 and 5), rat small intestine (lanes 2 and 6), rat liver (lanes 3 and 7) and mouse liver (lanes 4 and 8) lysates.1-4. Anti-Ferroportin (SLC40A1) Antibody (#ARA6618), (1:500). 5-8. Anti-Ferroportin (SLC40A1) Antibody, preincubated with Ferroportin/SLC40A1 Blocking Peptide .

Immunohistochemical staining of perfusion-fixed frozen mouse substantia nigra pars compacta (SNC) sections using Anti- Ferroportin (SLC40A1) Antibody (#ARA6618). A. Ferroportin staining (green) appears in several cells in the SNC. B. Tyrosine hydroxylase (red) stains dopaminergic neurons. C. Merge of A and B demonstrates Ferroportin in dopaminergic and non- dopaminergic neurons. Cell nuclei are stained using DAPI (blue).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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