FIH-1 Mouse Monoclonal Antibody(C4022)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:1000 - 1:2000 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to FIH-1 |
Specificity | Recognizes endogenous levels of FIH-1 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human FIH-1. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 40 kD; Observed: 40 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | FIH1; Hypoxia-inducible factor 1-alpha inhibitor; Factor inhibiting HIF-1; FIH-1; Hypoxia-inducible factor asparagine hydroxylase |
Gene Symbol | HIF1AN |
Entrez Gene | 55662(Human) |
SwissProt | Q9NWT6(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of FIH-1 expression in 293 (A), Jurkat (B), ZR751 (C) whole cell lysates. (Predicted band size: 40 kD; Observed band size: 40 kD)
Immunofluorescent analysis of FIH-1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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