Fibrinogen alpha chain Rabbit Monoclonal Antibody (ARA822)

Datasheet

MSDS

Fibrinogen alpha chain Rabbit Monoclonal Antibody (ARA822)

Key features and details

Target: Fibrinogen alpha chain
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IHC(P)
Storage: -20°C

Brand:

CAT.NO. : ARA6614

US$ Please choose

Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Fibrinogen alpha chain is a protein that in humans is encoded by the FGA gene. The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein composed of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin, which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia, and renal amyloidosis. Alternative splicing results in two isoforms that vary in the carboxy-terminus.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:100-1:2,000
IHC	1:500-1:1,000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Molecular Weight	Predicted band size: 95 kDa
Clonality	Monoclonal
Species Reactivity	Human, Mouse, Rat
Isotype	IgG
Host	Rabbit
Form/Format	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Purity/Purification	Purified
Immunogen	Synthetic peptide.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of Fibrinogen alpha chain on different lysates with Fibrinogen alpha chain Rabbit Monoclonal Antibody (ARA822) at 1/1,000 dilution.Lane 1: Human plasma lysate (30 µg/Lane)Lane 2: Mouse plasma lysate (30 µg/Lane)Lane 3: Rat plasma lysate (30 µg/Lane)Predicted band size: 95 kDaObserved band size: 100 kDaExposure time: 59 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ARA822) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta labeling Fibrinogen alpha chain with ARA822.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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