FAM120A Mouse Monoclonal Antibody(C4025)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IHC | 1:50 - 1:200 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to FAM120A |
Specificity | Recognizes endogenous levels of FAM120A protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human FAM120A. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 121 kD; Observed: 132 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | C9orf10; KIAA0183; OSSA; Constitutive coactivator of PPAR-gamma-like protein 1; Oxidative stress-associated Src activator; Protein FAM120A |
Gene Symbol | FAM120A |
Entrez Gene | 23196(Human) |
SwissProt | Q9NZB2(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of FAM120A expression in MCF7 (A), U2OS (B) whole cell lysates. (Predicted band size: 121 kD; Observed band size: 132 kD)
Immunohistochemical analysis of FAM120A staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of FAM120A staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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