Factor VIII Mouse Monoclonal Antibody(ARA969)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
-
Brand:
CAT.NO. : ARA6832
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Size:
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Product Details
Background
Factor VIII-R (Factor VIII related antigen or von Willebrand factor) is a multimeric glycoprotein that mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.
Factor VIII-R has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. It is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms.
Not all endothelial cells synthesize or store this molecule, so about 30% of tumors of vascular origin fail to stain for Factor VIII-R, regardless of whether they are benign or malignant. Staining for Factor VIII-R has also been used to measure angiogenesis, an indicator of tumor recurrence.
Factor VIII-R reacts specifically with the endothelial cells of normal, reactive, and neoplastic blood and lymphatic vessels, showing a finely granular cytoplasmic staining. It also reacts with endocardium, platelets, and megakaryocytes.
Factor VIII-R has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. It is one of the most useful markers to identify endothelial (or megakaryocytic) lineage of neoplasms.
Not all endothelial cells synthesize or store this molecule, so about 30% of tumors of vascular origin fail to stain for Factor VIII-R, regardless of whether they are benign or malignant. Staining for Factor VIII-R has also been used to measure angiogenesis, an indicator of tumor recurrence.
Factor VIII-R reacts specifically with the endothelial cells of normal, reactive, and neoplastic blood and lymphatic vessels, showing a finely granular cytoplasmic staining. It also reacts with endocardium, platelets, and megakaryocytes.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Species Reactivity | Humans; others not tested |
Known Applications | Immunohistochemistry (formalin-fixed, paraffin-embedded tissues) |
Supplied As | Buffer with protein carrier and preservative |
Control | Tonsil |
Visualization | Cytoplasmic |
Isotype | IgG1/κ |
Immunogen | Recombinant fragment of human vWF protein (aa 1815-1939) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human tonsil tissue sections using Factor VIII Mouse Monoclonal Antibody (ARA969).
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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