ERK7 Mouse Monoclonal Antibody(C3955)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to ERK7 |
Specificity | Recognizes endogenous levels of ERK7 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human ERK7. The exact sequence is proprietary. |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 59 kD; Observed: 59 kD |
Form/Buffer | Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8 |
Gene Symbol | MAPK15 |
Entrez Gene | 225689(Human) |
SwissProt | Q8TD08(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of ERK7 expression in MCF7 (A) whole cell lysates. (Predicted band size: 59 kD; Observed band size: 59 kD)
Immunofluorescent analysis of ERK7 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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