EN2 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:10 - 1:50 |
Description | Rabbit polyclonal antibody to EN2 |
Specificity | Recognizes endogenous levels of EN2 protein. |
Antibody Type | Primary antibody |
Imnunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human EN2. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 34 kD; Observed: 34 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Homeobox protein engrailed-2; Homeobox protein en-2; Hu-En-2 |
Gene Symbol | EN2 |
Entrez Gene | 2020(Human); 13799(Mouse) |
SwissProt | P19622(Human); P09066(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of EN2 expression in MCF7 (A) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)
Immunofluorescent analysis of Anti-EN2 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
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