eIF2S3 Rabbit Polyclonal Antibody

Datasheet

MSDS

Key features and details

Rabbit polyclonal antibody to eIF2S3

Target: eIF2S3
Source/Host: Rabbit
Reactivity: Human,Mouse,Rat
Clonality: Polyclonal
Applications: WB, IHC
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : APA12933

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Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA . This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form the 43S pre-initiation complex (43S PIC) . Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex . In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF-2B .

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:500 - 1:2000
IHC	1:50 - 1:200

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit polyclonal antibody to eIF2S3
Specificity	Recognizes endogenous levels of eIF2S3 protein.
Antibody Type	Primary antibody
Imnunogen	Recombinant fusion protein of human eIF2S3
Purification	The antibody was purified by immunogen affinity chromatography.
Molecular Weight	Predicted: 51 kD; Observed: 51 kD
Form/Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative Names	EIF2G; Eukaryotic translation initiation factor 2 subunit 3; Eukaryotic translation initiation factor 2 subunit gamma X; eIF-2-gamma X; eIF-2gX
Gene Symbol	EIF2S3
Entrez Gene	1968(Human); 26905(Mouse); 100312984; 299027(Rat)
SwissProt	P41091(Human); Q9Z0N1(Mouse); P81795(Rat)

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of eIF2S3 expression in SW620 (A), HL60 (B) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 51 kD)

Immunohistochemical analysis of eIF2S3 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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