EG5 Rabbit Monoclonal Antibody(C3240)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
Description | Rabbit monoclonal antibody to EG5 |
Specificity | Recognizes endogenous levels of EG5 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant protein of human Eg5 |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 119 kD; Observed: 119 kD |
Form/Buffer | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
Alternative Names | EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5 |
Gene Symbol | KIF11 |
Entrez Gene | 3832(Human); 16551(Mouse) |
SwissProt | P52732(Human); Q6P9P6(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of EG5 expression in NIH3T3 (A), MEF (B), Hela (C), A549 (D), HL60 (E), U2OS (F), C6 (G) whole cell lysates. (Predicted band size: 119 kD; Observed band size: 119 kD)
Immunofluorescent analysis of EG5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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