EG5 Mouse Monoclonal Antibody(C3279)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
IP | 1:10 - 1:50 |
Description | Mouse monoclonal antibody to EG5 |
Specificity | Recognizes endogenous levels of EG5 protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human Eg5 protein fragments expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 119 kD; Observed: 130 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | EG5; KNSL1; TRIP5; Kinesin-like protein KIF11; Kinesin-like protein 1; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5 |
Gene Symbol | KIF11 |
Entrez Gene | 3832(Human) |
SwissProt | P52732(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of EG5 expression in MCF7 (A), 293T (B), Jurkat (C), Hela (D) whole cell lysates. (Predicted band size: 119 kD; Observed band size: 130 kD)
Immunofluorescent analysis of EG5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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