Cytokeratin 8 Rabbit Monoclonal Antibody(ARA739)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:2500-1:5000 |
IHC | 1:100-1:200 |
IF/ICC | 1:5000-1:10000 |
FC | 1:200-1:800 |
IP | 1:30 |
Description | Rabbit Monoclonal Antibody to Cytokeratin 8 |
Antibody Type | Primary antibody |
Predicted MW | 53kDa |
Immunogen | A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 8 was used as an immunogen. |
Purification | ProA affinity purified IgG |
Form/Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.14%. |
Alternative Names | CYK8; Keratin, type II cytoskeletal 8; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8 |
Gene Symbol | KRT8 |
Entrez Gene | 3856(Human) |
Swissprot | P05787 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
All lanes: Anti-Cytokeratin 8 antibody at 1:5,000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
Lane 1: A431
Lane 2: HepG2
Lysate at 10 µg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 100s
All lanes: Anti-Cytokeratin 8 antibody at 1:2,000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
Lane 1: Hela
Lane 2: SW480
Lane 3: A549
Lysate at 10 µg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 100s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Cytokeratin 8 with ARA739 at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
ARA739 staining Cytokeratin 8 in Hela cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with primary antibody (1:10,000) at 4°C. An Alexa Fluor<sup>®</sup> 488-conjugated Goat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1:500). DAPI (blue) was used as the nuclear counter stain.
Control: PBS and secondary antibody, An Alexa Fluor<sup>®</sup> 488-conjugated Goat Anti-Rabbit IgG (1:500).
Overlay histogram showing Hela cells stained with ARA739 (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the antibody (ARA739, 1:800 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Black) was used as a control.
Cytokeratin 8 was immunoprecipitated from 0.4mg of A431 whole cell lysate with ARA739 at 1:30 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA739 IP in A431 whole cell lysate
Lane 2: PBS instead of ARA739 in A431 whole cell lysate
Lane 3: A431 whole cell lysate, 10 µg (input)
Exposure: 20s
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