Cytokeratin 7 Mouse Monoclonal Antibody(C3296)
Datasheet 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
Description | Mouse monoclonal antibody to Cytokeratin 7 |
Specificity | Recognizes endogenous levels of Cytokeratin 7 protein. |
Antibody Type | Primary antibody |
Imnunogen | Purified recombinant human Keratin 7 protein fragments expressed in E.coli. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 51 kD; Observed: 55 kD |
Form/Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
Alternative Names | SCL; Keratin type II cytoskeletal 7; Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7 |
Gene Symbol | KRT7 |
Entrez Gene | 3855(Human) |
SwissProt | P08729(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Cytokeratin 7 expression in T47D (A), Hela (B), HepG2 (C) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 55 kD)
Immunofluorescent analysis of Cytokeratin 7 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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