Cytokeratin 17 Rabbit Monoclonal Antibody(ARB936)
Datasheet 
Key features and details
- Target:
- Clone ID:
- Source/Host:
- Reactivity:
- Applications:
- Dilution:
- Clonality:
- Storage:
-
Brand:
CAT.NO. : ARB6728
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Product Details
Background
Cytokeratin 17 (CK17) is 48 kD intermediate filament found in simple epithelia and is a marker for cervical reserve (stem) cells. CK17 can be useful in the identification of uterine cervical squamous lesions including atypical immature metaplasia, cervical intraepithelial neoplasia, and cervical squamous carcinoma. CK17 also assists in the differentiation of oral squamous cell carcinoma. CK17 staining occurs in human epithelial appendages such as hair follicles. Studies indicate CK17 maybe an excellent marker for the identification of squamous cell carcinomas in various tissues including the cervix, lung and oral cavity. CK17 may also be helpful in distinguishing myoepithelial cells from luminal epithelium of various glands such as mammary, sweat and salivary. Positive expression of CK17 in breast cancer has been associated with a worse prognosis, high tumor grade and positive axillary lymph nodes. Expressed in the outer root sheath and medulla region of hair follicle specifically from eyebrow and beard, digital pulp, nail matrix and nail bed epithelium, mucosal stratified squamous epithelia and in basal cells of oral epithelium, palmoplantar epidermis and sweat and mammary glands. Also expressed in myoepithelium of prostate, basal layer of urinary bladder, cambial cells of sebaceous gland and in exocervix (at protein level).
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:100 - 1:200 |
Overview
Predicted Molecular Wt | 48kDa |
Purity | ProA affinity purified IgG |
Subcellular location | Cytoplasmic |
Swissprot ID | Q04695 |
Immunogen | Synthetic peptide corresponding to residues within aa400-500 of Human Cytokeratin 17 |
Storage Buffer | PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.05%. |
Recommended method | Heat induced epitope retrieval with Tris-EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C-25°C) for 30 minutes. |
Alternative Names | Keratin, type I cytoskeletal 17 (39.1) (Cytokeratin-17) (CK-17) (Keratin-17) (K17) |
Gene Symbol | KRT17 |
Entrez Gene | 3872 |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical staining of human esophagus carcinoma tissue using Cytokeratin 17 Rabbit Monoclonal Antibody (ARB936).
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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