Cytokeratin 16 Rabbit Monoclonal Antibody(ARA855)

Datasheet

MSDS

Cytokeratin 16 Rabbit Monoclonal Antibody(ARA855)

Key features and details

Target: Cytokeratin 16
Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Application: IHC
Storage: -20°C

Brand:

CAT.NO. : ARA6681

US$ Please choose

Size:

1mL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN).

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
IHC	1:50-1:1,500

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Antibody Type	Recombinant Rabbit monoclonal Antibody
Immunogen	Synthetic peptide within human Cytokeratin 16 aa 27 - 75 / 473
Molecular Weight	Predicted band size: 51 kDa
Positive Control	HeLa cell lysate, HaCaT cell lysate, HepG2 cell lysate, MCF7 cell lysate, A431, human esophagus tissue, human tonsil tissue, human lung carcinoma tissue, human breast carcinoma tissue, human breast tissue
Conjugation	unconjugated
Form/Buffer	1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
Isotype	IgG
Purification	Protein A affinity purified

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Immunohistochemical analysis of paraffin-embedded human esophagus tissue with Cytokeratin 16 Rabbit Monoclonal Antibody(ARA855).

Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Cytokeratin 16 Rabbit Monoclonal Antibody(ARA855).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using Cytokeratin 16 Rabbit Monoclonal Antibody(ARA855).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Cytokeratin 16 Rabbit Monoclonal Antibody(ARA855).

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?

AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.

What development scenarios are pathology raw antibodies mainly used for?

Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.

How can I evaluate whether a raw antibody is suitable for our staining platform?

It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.

Can you provide samples for platform validation?

Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.

How should raw antibodies be paired with detection systems?

AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.

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