CTAG1A/NY-ESO-1 Rabbit Monoclonal Antibody
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA6621
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Size:
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Product Details
Background
CTAG1B (cancer/testis antigen 1), also known as NY-ESO-1 (Autoimmunogenic cancer/testis antigen) is an 18 kDa protein with putative roles in germ cell self-renewal and/or differentiation. Of cancer/testis (CT) antigens, CTAG1B is the most immunogenic CT antigen known to date. CTAG1B staining is predominantly cytoplasmic with focal nuclear expression. Tissue distribution is highly restricted in the normal adult; it is only detected in spermatogonia and primary spermatocytes within the testis. Surrounding non-gametogenic cells, including Sertoli cells and spermatids are negative. CTAG1B is also undetected in the ovaries. However, expression is observed in germ cells of the fetal testis and ovaries. In cancer, genome wide demethylation was shown to induce CTAG1B expression. Immunohistochemical expression has been demonstrated commonly in myxoid and round cell liposarcoma (89-100%), neuroblastoma (82%), synovial sarcoma (80%), melanoma (46%) and epithelial ovarian cancer (43%). Staining in lung, esophageal, liver, gastric, and bladder cancers has also been noted with lower prevalence. Immunoreactivity was only observed in tumor cells; no staining was present in adjacent cells. Furthermore, previous studies have correlated CTAG1B antigen expression with advance stage of disease and indicated a subset of more aggressive tumors that may be less susceptible to known treatments. CTAG1B antibody has been used for identification of myxoid and round cell liposarcoma.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
IHC | 1:1,000-1:2,000 |
Overview
Antibody Type | Recombinant Rabbit monoclonal Antibody |
Immunogen | Recombinant full length protein corresponding to Human CTAG1B |
Molecular Weight | Predicted band size: 18 kDa |
Positive Control | Human testis tissue, human stomach carcinoma tissue |
Conjugation | unconjugated |
Form/Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide |
Isotype | IgG |
Purification | Protein A affinity purified |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immunohistochemical analysis of paraffin-embedded human testis tissue with CTAG1A/NY-ESO-1 Rabbit Monoclonal Antibody.
Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue with CTAG1A/NY-ESO-1 Rabbit Monoclonal Antibody.
Storage
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
Are the pathology antibodies provided by AREX raw antibodies or ready-to-use solutions?
AREX Biosciences specializes in supplying high-quality IHC pathology raw antibodies (Raw Antibodies). We do not manufacture ready-to-use working solutions or IVD diagnostic reagents. We mainly provide concentrated raw materials to pathology reagent manufacturers and diagnostic platform companies to support product development and OEM production.
What development scenarios are pathology raw antibodies mainly used for?
Our raw antibodies are primarily used for the research, performance optimization, validation, and commercial-scale production of pathology IHC detection reagents. They are also suitable for companion diagnostics (CDx) projects and antibody screening.
How can I evaluate whether a raw antibody is suitable for our staining platform?
It is recommended to focus on specificity, sensitivity, background control, and performance on your target automated platforms (such as Roche Ventana, Leica Bond, Agilent Dako, etc.). We can provide internal test data for reference, but we recommend partners perform actual validation on their own platforms.
Can you provide samples for platform validation?
Yes. We can provide validation samples depending on the specific product. Some products may be provided free of charge, while others may involve a small sample fee. Handling fee and shipping fee will be charged separately. Please contact us for detailed confirmation.
How should raw antibodies be paired with detection systems?
AREX raw antibodies can be used with various polymer detection systems and ancillary reagents. We recommend optimization in combination with the ARExVisual® system or your existing detection platform to achieve better signal intensity and background control.
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