COVID-19 S-IgM Neutralizing Human Cells Monoclonal Antibody(C2478)
MSDS 
Key features and details
Human monoclonal neutralizing antibody to COVID-19 S protein
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : AMA02090
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Product Details
Background
Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2 . When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane . When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane . Alternatively, may use NRP1/NRP2 and integrin as entry receptors . The use of NRP1/NRP2 receptors may explain the tropism of the virus in human olfactory epithelial cells, which express these molecules at high levels but ACE2 at low levels . Uses also ASGR1 as an alternative receptor in an ACE2-independent manner .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
ELISA | Use at an assay dependent dilution. |
Overview
Description | Human monoclonal neutralizing antibody to COVID-19 S protein |
Specificity | Recognizes COVID-19 S Protein, COVID-19 S-RBD Protein and block ACE-2 receptor binding. |
Antibody Type | Primary antibody |
Imnunogen | |
Purification | >95%, cultured in vitro under conditions free from animal derived components. |
Molecular Weight | N/A |
Form/Buffer | 1 mg/ml. Human IgM. Liquid in 0.01M Phosphate Buffered Saline, pH 7.4. |
Alternative Names | Spike glycoprotein; S glycoprotein; E2; Peplomer protein |
Gene Symbol | S |
Entrez Gene | 43740568(Human) |
SwissProt | P0DTC2(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Immobilized Recombinant COVID-19 S Protein RBD-SD1 at 5 ug/ml can bind Anti-COVID-19 S-IgM Neutralizing Antibody, the EC50 is less than 18.5 ng/ml.
Anti-COVID-19 S-IgM Neutralizing Antibody can block Recombinant Human ACE2 Protein and Recombinant COVID-19 S Protein RBD-SD1 interaction, the IC50 for this effect is 83.9 ng/ml.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for use in diagnostic procedures.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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