COMMD5 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to COMMD5 |
Specificity | Recognizes endogenous levels of COMMD5 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant protein of human COMMD5. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 24 kD; Observed: 25 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | COMM domain-containing protein 5; Hypertension-related calcium-regulated gene protein; HCaRG; HT002 |
Gene Symbol | COMMD5 |
Entrez Gene | 28991(Human) |
SwissProt | Q9GZQ3(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of COMMD5 expression in HeLa (A), mouse kidney (B), rat liver (C) whole cell lysates. (Predicted band size: 24 kD; Observed band size: 25 kD)
Immunofluorescent analysis of COMMD5 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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