Collagen 3 alpha 1 Rabbit Monoclonal Antibody(C3136)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
IP | 1:10 - 1:50 |
Description | Rabbit monoclonal antibody to Collagen 3 alpha 1 |
Specificity | Recognizes endogenous levels of Collagen 3 alpha 1 protein. |
Antibody Type | Primary antibody |
Imnunogen | Recombinant protein of human Collagen III |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 139 kD; Observed: 150 kD |
Form/Buffer | Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
Alternative Names | Collagen alpha-1(III) chain |
Gene Symbol | COL3A1 |
Entrez Gene | 1281(Human); 12825(Mouse); 84032(Rat) |
SwissProt | P02461(Human); P08121(Mouse); P13941(Rat) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Collagen 3 alpha 1 expression in K562 (A), C6 (B), NIH3T3 (C) whole cell lysates. (Predicted band size: 139 kD; Observed band size: 150 kD)
Immunofluorescent analysis of Collagen 3 alpha 1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark.
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