Collagen 2 alpha 1 Mouse Monoclonal Antibody(C2647)
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:100 |
FC | 1:100 - 1:200 |
Description | Mouse monoclonal to Collagen 2 alpha 1 |
Specificity | Recognizes endogenous levels of Collagen 2 alpha 1 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human Collagen 2 alpha 1 expressed in HEK293 |
Purification | This antibody is purified through a protein G column. |
Molecular Weight | Predicted: 142 kD; Observed: 130 kD kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | Collagen alpha-1(II) chain; Alpha-1 type II collagen |
Gene Symbol | COL2A1 |
Entrez Gene | 1280(Human) |
SwissProt | P02458(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of Collagen 2 alpha 1 expression in Hela (A), MCF7 (B), A549 (C), Jurkat (D), K562 (E) whole cell lysates. (Predicted band size: 142 kD; Observed band size: 130 kD kD)
Immunofluorescent analysis of Collagen 2 alpha 1 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with an AREX® Fluor 488 -conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AREX® Fluor 594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of Jurkat cells using Anti-Collagen 2 alpha 1 Antibody (green) and negative control (red).
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