claudin-1 Mouse Monoclonal Antibody (XX7)
Datasheet 
Key features and details
- Target:
- Host:
- Reactivity:
- Clonality:
- Application:
- Storage:
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Brand:
CAT.NO. : ARA0127
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Product Details
Background
The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction - bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. Claudin expression is often highly restricted to specific regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin - 1 is a multi - pass membrane protein that is expressed at high levels in kidney and liver and at lower levels in spleen, heart, brain, lung and testis. Defects in the gene encoding claudin - 1 are the cause of ichthyosis - sclerosing cholangitis neonatal syndrome (NISCH), an autosomal recessive syndrome characterized by vulgar type ichthyosis, scalp hypotrichosis, scarring alopecia and sclerosing cholangitis.
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
Application | Dilution Ratio |
WB | 1:100-1:1000 |
IP | 1-2 µg/100-500 µg total protein |
IF | 1:50-1:500 |
IHC | 1:50-1:500 |
ELISA | 1:30-1:3000 |
Overview
Isotype | Mouse IgG1 |
Target Antigen | Claudin-1 |
Gene Name | CLDN1 |
UniProt ID | O95832 (Human), O88551 (Mouse), P56745 (Rat) |
Entrez Gene ID | 9076 (Human), 12737 (Mouse), 65129 (Rat) |
Molecular Weight | 22 kDa |
Gene Aliases | CLD1, ILVASC, SEMP1, Claudin 1 |
Immunogen | Recombinant Claudin-1 of human origin |
Form/Buffer | PBS with < 0.1% sodium azide and 0.1% gelatin |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of claudin - 1 expression in non - transfected 293T (A), human claudin - 1 transfected 293T (B) and SCC - 4 (C) whole cell lysates.
Immunoperoxidase staining of formalin - fixed, paraffin - embedded human stomach tissue showing membrane and cytoplasmic localization (A).
Storage
Store at 4°C, DO NOT FREEZE. Stable for one year from the date of shipment.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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