CGRP Rabbit Monoclonal Antibody(ARA667)

Datasheet

MSDS

Key features and details

Target: CGRP
Host: Rabbit
Reactivity: Human, Mouse, Rat
Clonality: Monoclonal
Application: WB, IHC, IF/ICC, FC
Storage: -20°C

Brand:

CAT.NO. : ARA6385

US$ Please choose

Size:

50μL 100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Calcitonin gene-related peptide (CGRP) is a member of the calcitonin family of peptides consisting of calcitonin, amylin, adrenomedullin, adrenomedullin 2 (intermedin) and calcitonin‑receptor‑stimulating peptide. CGRP is produced in both peripheral and central neurons. It is a potent peptide vasodilator and can function in the transmission of nociception. In the spinal cord, the function and expression of CGRP may differ depending on the location of synthesis. CGRP is derived mainly from the cell bodies of motor neurons when synthesized in the ventral horn of the spinal cord and may contribute to the regeneration of nervous tissue after injury. Conversely, CGRP is derived from dorsal root ganglion when synthesized in the dorsal horn of the spinal cord and may be linked to the transmission of pain. In the trigeminal vascular system, the cell bodies of the trigeminal ganglion are the main source of CGRP. CGRP is thought to play a role in cardiovascular homeostasis and nociception. In the heart, CGRP acts as a chronotrope by increasing heart rate. Apart from these attributes, CGRP is known to modulate the autonomic nervous system and plays a role in ingestion. CGRP has moderate effects on calcium homeostasis compared to its extensive actions in other areas, such as the autonomic nervous system.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

Application	Dilution Ratio
WB	1:1000-1:2000
IHC	1:200-1:2000
IF/ICC	1:100-1:200
FC	1:1000-1:10000

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Product Description	Recombinant Rabbit monoclonal Antibody to CGRP
Immunogen	Recombinant protein within rat CGRP aa 1-128.
Clonality	Monoclonal
Purification	Protein A affinity purified.
Isotype	IgG
Conjugate	unconjugated
Form	Liquid
Form/Buffer	PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Reactivity Species	Human, Mouse, Rat
Molecular Weight	Predicted band size: 14 kDa
Positive Control	TT cell lysate, human brain tissue, mouse spinal cord tissue, rat spinal cord tissue, mouse colon tissue, rat colon tissue, TT.

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

Western blot analysis of CGRP on different lysates with CGRP Rabbit Monoclonal Antibody(ARA667) at 1/2,000 dilution.

Immunohistochemical analysis of paraffin-embedded human brain tissue with CGRP Rabbit Monoclonal Antibody(ARA667) at 1/200 dilution.

Immunocytochemistry analysis of TT (positive) and HeLa (negative) labeling CGRP with CGRP Rabbit Monoclonal Antibody(ARA667) at 1/100 dilution.

Flow cytometric analysis of HeLa (left, negative) and TT (right, positive) cells labeling CGRP.

Storage

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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