CGRP Rabbit Monoclonal Antibody(ARA667)
Key features and details
- Reactivity:
- Application:
- Host:
- Clonality:
- Target:
-
Brand:
CAT.NO. : ARA6385
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Product Details
Background
Calcitonin gene-related peptide (CGRP) is a member of the calcitonin family of peptides consisting of calcitonin, amylin, adrenomedullin, adrenomedullin 2 (intermedin) and calcitonin‑receptor‑stimulating peptide. CGRP is produced in both peripheral and central neurons. It is a potent peptide vasodilator and can function in the transmission of nociception. In the spinal cord, the function and expression of CGRP may differ depending on the location of synthesis. CGRP is derived mainly from the cell bodies of motor neurons when synthesized in the ventral horn of the spinal cord and may contribute to the regeneration of nervous tissue after injury. Conversely, CGRP is derived from dorsal root ganglion when synthesized in the dorsal horn of the spinal cord and may be linked to the transmission of pain. In the trigeminal vascular system, the cell bodies of the trigeminal ganglion are the main source of CGRP. CGRP is thought to play a role in cardiovascular homeostasis and nociception. In the heart, CGRP acts as a chronotrope by increasing heart rate. Apart from these attributes, CGRP is known to modulate the autonomic nervous system and plays a role in ingestion. CGRP has moderate effects on calcium homeostasis compared to its extensive actions in other areas, such as the autonomic nervous system.
Application
|
Application |
Dilution Ratio |
|
WB |
1:1000-1:2000 |
|
IHC |
1:200-1:2000 |
|
IF/ICC |
1:100-1:200 |
|
FC |
1:1000-1:10000 |
Overview
|
Product Description |
Recombinant Rabbit monoclonal Antibody to CGRP |
|
Immunogen |
Recombinant protein within rat CGRP aa 1-128. |
|
Clonality |
Monoclonal |
|
Purification Method |
Protein A affinity purified. |
|
Isotype |
IgG |
|
Conjugate |
unconjugated |
|
Form |
Liquid |
|
Storage Buffer |
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
|
Reactivity Species |
Human, Mouse, Rat |
|
Molecular Weight |
Predicted band size: 14 kDa |
|
Positive Control |
TT cell lysate, human brain tissue, mouse spinal cord tissue, rat spinal cord tissue, mouse colon tissue, rat colon tissue, TT. |
Data
Western blot analysis of CGRP on different lysates with CGRP Rabbit Monoclonal Antibody(ARA667) at 1/2,000 dilution.
Immunohistochemical analysis of paraffin-embedded human brain tissue with CGRP Rabbit Monoclonal Antibody(ARA667) at 1/200 dilution.
Immunocytochemistry analysis of TT (positive) and HeLa (negative) labeling CGRP with CGRP Rabbit Monoclonal Antibody(ARA667) at 1/100 dilution.
Flow cytometric analysis of HeLa (left, negative) and TT (right, positive) cells labeling CGRP.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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