CEP85 Rabbit Polyclonal Antibody
MSDS 
Key features and details
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
Product Details
WB | 1:500 - 1:2000 |
IF/ICC | 1:50 - 1:200 |
Description | Rabbit polyclonal antibody to CEP85 |
Specificity | Recognizes endogenous levels of CEP85 protein |
Antibody Type | Primary antibody |
Imnunogen | Recombinant fusion protein of human CEP85. The exact sequence is proprietary. |
Purification | The antibody was purified by immunogen affinity chromatography. |
Molecular Weight | Predicted: 22; Observed: 105 kD |
Form/Buffer | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Alternative Names | CCDC21; Centrosomal protein of 85 kDa; Cep85; Coiled-coil domain-containing protein 21 |
Gene Symbol | CEP85 |
Entrez Gene | 64793(Human); 70012(Mouse) |
SwissProt | Q6P2H3(Human); Q8BMK0(Mouse) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Western blot analysis of CEP85 expression in Jurkat (A), HeLa (B) whole cell lysates. (Predicted band size: 22; 80; 85 kD; Observed band size: 105 kD)
Immunofluorescent analysis of CEP85 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AREX® Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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