CD9 Mouse Monoclonal Antibody(HI9a)
Datasheet 
Key features and details
Mouse monoclonal antibody to CD9
- Target:
- Source/Host:
- Reactivity:
- Clonality:
- Applications:
- Conjugation:
- Storage:
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Brand:
CAT.NO. : AMA03794
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Product Details
Background
Integral membrane protein associated with integrins, which regulates different processes, such as sperm-egg fusion, platelet activation and aggregation, and cell adhesion . Present at the cell surface of oocytes and plays a key role in sperm-egg fusion, possibly by organizing multiprotein complexes and the morphology of the membrane required for the fusion . In myoblasts, associates with CD81 and PTGFRN and inhibits myotube fusion during muscle regeneration . In macrophages, associates with CD81 and beta-1 and beta-2 integrins, and prevents macrophage fusion into multinucleated giant cells specialized in ingesting complement-opsonized large particles . Also prevents the fusion between mononuclear cell progenitors into osteoclasts in charge of bone resorption .
Application
To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.
*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.
WB | 1:500 - 1:1000 |
IF/ICC | 1:50 - 1:200 |
FC | 1:500 - 1:2000 |
Overview
Description | Mouse monoclonal antibody to CD9 |
Specificity | Recognizes human CD9 |
Antibody Type | Primary antibody |
Imnunogen | Native purified human CD9. |
Purification | The antibody was purified by affinity chromatography. |
Molecular Weight | Predicted: 25 kD; Observed: 24 kD |
Form/Buffer | Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide. |
Alternative Names | MIC3; TSPAN29; CD9 antigen; 5H9 antigen; Cell growth-inhibiting gene 2 protein; Leukocyte antigen MIC3; Motility-related protein; MRP-1; Tetraspanin-29; Tspan-29; p24; CD antigen CD9 |
Gene Symbol | CD9 |
Entrez Gene | 928(Human) |
SwissProt | P21926(Human) |
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.
Data
Western blot analysis of CD9 expression in mouse brain (A), mouse lung (B) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 24 kD)
Flow cytometric analysis of human platelet using Anti-CD9 Antibody, followed by anti-mouse IgG PE.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Note
For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.
FAQs
What are the main types of research antibodies and how do they differ?
Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.
How can I tell if a research antibody is suitable for my experiment?
It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.
Can improper storage of research antibodies affect experimental results?
Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.
Why doesn’t the recommended dilution in the datasheet work well in my experiment?
The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.
What precautions should I take when using a newly purchased research antibody for the first time?
It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.
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