CD4 Rabbit Monoclonal Antibody(ARA818)

Datasheet

MSDS

Key features and details

Target: CD4
Source/Host: Rabbit
Reactivity: Human
Clonality: Monoclonal
Applications: WB,IHC,FC,IP
Conjugation: Unconjugated
Storage: at-20°C

Brand:

CAT.NO. : ARA6608

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Size:

100μL

Trail, Bulk size or Custom requests Please contact us

Product Details

Background

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex.

Application

To ensure optimal assay performance, AREX recommends conducting reagent titration tailored to each testing system for optimal detection results.

WB	1:2000-1:5000
IHC	1:1000-1:2000
FC	1:10-1:50
IP	1:50

*Results are sample-specific. Please refer to your local assay conditions and test parameters for reference.

Overview

Description	Rabbit Monoclonal Antibody to CD4
Antibody Type	Primary antibody
Predicted MW	51kDa
Immunogen	Recombinant protein corresponding to aa200-400 of human CD4 was used as an immunogen.
Purification	ProA affinity purified IgG
Form/Buffer	PBS 59%, Sodium azide 0.01%, Glycerol 40%, BSA 0.93%.
Alternative Names	T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD4
Gene Symbol	CD4
Entrez Gene	920(Human)
Swissprot	P01730

*AREX continuously optimizes our products. Webpage content may not reflect the latest updates. For inquiries, please contact info@arexbio.com or your local distributor.
*Clone Number, Reactivity, Source/Host and Clonality can be found in the product name and Key Features section above.

Data

All lanes: Anti-CD4 antibody at 1:2,000 dilution
Predicted MW: 51 kDa
Observed MW: 51 kDa
Lane 1: Molt-4
Lane 2: THP-1
Lane 3: HuT-78
Lysate at 10 µg per lane
2nd Ab:
G&R HRP(H+L) 1:10,000
Exposure: 100s

CD4 was immunoprecipitated from 0.4mg of Molt-4 whole cell lysate with ARA818 at 1:50 dilution.
2nd Ab:
GAR HRP for IP 1:500
Lane 1: ARA818 IP in Molt-4 whole cell lysate
Lane 2: Rabbit IgG instead of RR648 in Molt-4 whole cell lysate
Lane 3: Molt-4 whole cell lysate, 10 µg (input)
Exposure: 120s

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling CD4 with ARA818 at 1:2,000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.

Overlay histogram showing Jurkat cells stained with ARA818 (Blue). The cells were fixed with 4% paraformaldehyde for 10 min. The cells were then incubated in the antibody (ARA818, 1:50 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody used was a Goat Anti-Rabbit Alexa Fluor<sup>®</sup> 488 (IgG H+L) at 1:2,000 dilution for 20 min at room temperature. Unlabelled sample (Red) was used as a control.

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Note

For Research Use Only. Not for diagnostic, therapeutics, prophylactic or in vivo use.

FAQs

What are the main types of research antibodies and how do they differ?

Research antibodies are mainly divided into monoclonal antibodies and polyclonal antibodies. Monoclonal antibodies typically offer higher specificity and better batch-to-batch consistency, while polyclonal antibodies often provide stronger affinity but may show more variation between batches. The choice depends on your specific experimental needs.

How can I tell if a research antibody is suitable for my experiment?

It is recommended to carefully review the product datasheet for validated applications, species reactivity, recommended dilutions, and published references. For new antibodies, performing a small-scale validation with positive control samples is usually helpful.

Can improper storage of research antibodies affect experimental results?

Yes. Antibodies are sensitive to temperature, repeated freeze-thaw cycles, and contamination. Improper storage may lead to reduced activity, increased background, or weaker signals. It is best to follow the storage instructions provided in the product datasheet.

Why doesn’t the recommended dilution in the datasheet work well in my experiment?

The recommended dilution is based on the supplier’s test conditions. Factors such as sample type, fixation method, and detection system in your lab can influence the optimal working concentration. Performing a dilution series optimization in your own system is often necessary.

What precautions should I take when using a newly purchased research antibody for the first time?

It is advisable to briefly centrifuge the antibody (especially concentrated or lyophilized ones), then perform a small-scale pilot experiment using the recommended conditions. Recording the batch number and usage date is also helpful for future tracking.

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